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Overview | |
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Description | An exceptional MMP-2 (gelatinase A) and MMP-9 (gelatinase B) substrate this peptide is used for the continuous spectrophotometric assay of MMP-2 and MMP-9 in combination with a color-developing thiol-reactive agent, 4,4 €™-dithiodipyridine or Ellman €™s Reagent (as indicators). This thiol peptide-enzyme reaction has a Km of 0.004 M and a kcat of 103 s-1. Using this substrate, collagenase can be detected at concentrations as low as 2 ng/mL. HPLC and tandem mass spectrometry are also used to analyze MMP-2 and MMP-9 in conjunction with this peptide substrate. |
Sequence | Ac-PLG-SCH[CH2CH(CH3)2]-CO-LG-OC2H5 |
Sequence (3 Letter) | Ac - Pro - Leu - Gly - SCH[CH2CH(CH3)2] - CO - Leu - Gly - OC2H5 |
Molecular Weight | 655.9 |
Properties | |
Purity | % Peak Area By HPLC ≥ 95% |
Storage | -20 °C |
InnoPep Inc. is a company started by a couple of researchers with decades of expertise in peptide synthesis and conjugation aimed at enabling.