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Cathepsin D and E FRET Substrate
Mca - GKPILFFRLK(Dnp) - r - NH2
| Overview | |
|---|---|
| Description | An internally quenched fluorogenic substrate (Ab/Em =328/393 nm) for cathepsins D and E and not for B, H or L, obtained from the hepatopancreas (liver) of the Japanese common squid (Todarodes pacificus). The cleavage occurs at the Phe-Phe amide bond resulting in enhanced fluorescence and is used in screening cathepsin D and E inhibitors and for determining cathepsin D and E activity in tissue cell extracts. |
| Sequence | Mca-GKPILFFRLK(Dnp)-r-NH2 |
| Sequence (3 Letter) | 7 - Methoxycoumarin - 4 - Acetyl - Gly - Lys - Pro - Ile - Leu - Phe - Phe - Arg - Leu - Lys(Dnp) - D - Arg - NH2 |
| Molecular Weight | 1756.1 |
| Properties | |
| Purity | % Peak Area By HPLC ≥ 95% |
| Storage | -20 °C |
Yasuda, Y. et al. J. Biochem. 125, 1137 (1999).
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