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HCV Protease FRET Substrate (RET S1)
| Overview | |
|---|---|
| Description | This peptide is a HCV protease substrate incorporating an ester bond between residues P1 and P1. Due to ready transesterification of the scissile bond to the acyl-enzyme intermediate, this substrate shows very high kcat/Km values, enabling detection of activity with subnanomolar nonstructural protein 3 (NS3 protease) concentrations. It is widely used for the continuous assay of NS3 protease activity. Substrate cleavage is proportional to the enzyme concentration with a detection limit for NS3 between 1 nM and 250 pM. Upon cleavage of this substrate, fluorescence can be monitored at Abs/Em = 355/500 nm. Click here to see a flyer. |
| Sequence | Ac-DE-D(Edans)-EE-Abu-ψ |
| Sequence (3 Letter) | -[COO]-AS-K(Dabcyl)-NH2 |
| Molecular Weight | 1548.6 |
| Properties | |
| Purity | > 95% By HPLC |
| Storage | Store at -20 °C, cap vial tightly at all times. |
Lin, C. et al. J. Biol. Chem. 10, 1074 (2004); Kakiuchi, N. et al. J. Virol. Methods 80, 77 (1999); Liu, Y. et al. Anal. Biochem. 267, 331 (1999); Taliani, M. et al. Anal. Biochem. 240, 60 (1996).
About InnoPep
InnoPep Inc. is a company started by a couple of researchers with decades of expertise in peptide synthesis and conjugation aimed at enabling.